The second step at which ubiquitination or proteasome action may be implicated is after internalization, in routing of the receptor from early endocytic to late degradation compartments.
The Ubiquitin Proteasome Pathway (UPP) | Boston Biochem
Interestingly, ubiquitination of proteins can be regulated by specific ubiquitin proteases Wilkinson, In this respect, it is worth noting that one of these deubiquitinating enzymes named DUB-2 is induced by IL2 and down-regulated after the initiation of T-cell activation Zhu et al. However, we observed that, in the presence of the proteasome inhibitor, the extent of colocalization of two markers of normally distinct compartments, TfR and Lamp-1, was increased.
Effect of proteasome malfunction in the overall defect of vacuolar integrity was also one of the models proposed for the proteasome-dependent degradation of the a-factor transporter in yeast Loayza and Michaelis, Interestingly, a link between ubiquitin system and maintenance of intracellular integrity has also previously been described Lenk et al. In this report, it was shown, using ts20 mutant cell line, that a functional ubiquitination machinery is necessary for the maturation of autophagic vacuoles.
The role of proteasome in the routing of membrane proteins from the endocytic to degradation compartments is unclear.
One possible mechanism is that proteasome function might be involved in the regulation of a protein playing a crucial role in trafficking through the endocytic pathway. One potential candidate might be the recently described sorting nexin SNX15 Barr et al. Proteasomal degradation of some mammalian receptors has previously been described. For the platelet-derived growth factor Mori et al. We are grateful to Raymond Hellio and Pascal Roux for help with confocal microscopy, Annick Dujeancourt for skillfull technical assistance. The confocal microscope was purchased with a donation from Marcel and Liliane Pollack.
This work was supported by the Association pour la Recherche sur le Cancer no.
N -acetyl- l - l -leucyl-norleucinal. Molecular Biology of the Cell Vol. Anna Rocca Search for more papers by this author. E-mail address: E-mail Address: adautry pasteur. Add to favorites Download Citations Track Citations. Abstract Down-regulation of cell surface growth factor receptors plays a key role in the tight control of cellular responses.
Overexpression of a novel sorting nexin, SNX15, affects endosome morphology and protein trafficking. Traffic 1 , Structural design and molecular evolution of a cytokine receptor superfamily.
The Role of Proteasome in Apoptosis
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A Chinese hamster cell cycle mutant arrested at G2 phase has a temperature-sensitive ubiquitin-activating enzyme, E1. The Gap junction protein connexin43 is degraded via the ubiquitin proteasome pathway. Proteasome inhibitors: valuable new tools for cell biologists. Ubiquitin-activating enzyme, E1, is associated with maturation of autophagic vacuoles. Role for the ubiquitin-proteasome system in the vacuolar degradation of Ste6p, the a -factor transporter in Saccharomyces cerevisiae. Monoubiquitination is sufficient to signal internalization of the maltose transporter in Saccharomyces cerevisiae.
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Ozols , Amit Choudhury , Richard E. Pagano , and John R. Dean E. Submitted: 12 December Close Figure Viewer.
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